DIY Penicilin

Let's suppose every system collapse in a city, and you need to make antibiotics to treat phneumonia... I remember reading a while ago about someone that survived a civil war in an isolated country (middle east) Any idea how to make penicilin? I guess there should be an easy way to synthesize at least one dose of it.

>>8170 I don't know exactly why you want to make a penicillin but I was curious and did some research and found these steps: 1) Sterilize a container and lid using a pressure cooker or by cooking in an oven at 315℉ (157.222℃) for one hour. 2) Sterilize the growth medium for mold as much as possible. E.g; You can boil a pristine lemon, bake moist bread, or disinfect the fruit with alcohol. 3) Add the coarse bread or fruit. Place a piece of mold on the surface and close the lid of the jar. 4) Wait a few days for the mold to grow. Keep away from direct sunlight. Penicillium produces penicillin when the colony begins to mature and is under stress. Mold is most useful when it has reached the blue-green stage. Yes, you now have a Penicillium mold. So how do we extract this penicillin essence? One way is to add a weak acid (citric acid, cream of tartar, vitamin C) and water to the mold. Mix. Strain through the coffee filter and remove the liquid. The liquid contains diluted penicillin. Actually, it's not really necessary to purify penicillin. The mold itself is not toxic, further purification does not change its effect. If you prefer something other than purification, you can do the following: -Eat the mold. -Make a tea by mixing moldy bread or fruit with warm water. - If you are using moldy lemons, make lemon cream. VERY IMPORTANT!!: Remember that there are several types of this mold, and not all of them produce penicillin. That's why you need to distinguish Penicillum after obtaining a moldy product. Penicillium colony starts out as gray or white, turns blue and eventually turns blue-green. It typically develops a white outer ring. Aspergillus is another type of mold that resembles Penicillium. Aspergillus species can be green, gray or black. Some congeners of this mold also have commercial value, such as fermenting sake and producing citric acid. However, others cause disease or produce lethal poisons such as aflatoxin. You don't want to accidentally purify one of these.

>>8172 Probenecid will also increase the concentration and half-life of the penicillin, as well as whatever else OP cultures in his basement.

There are a ton of patents for penicillin synthesis that you can read through to learn the different methods other people have used over the years. Same goes for most medicine. >>8172 I got excited to try this and then remembered I'm allergic to penicillin.

It's not that difficult to find papers that discuss synthesis of antibiotics. https://www.nature.com/articles/ja2012126

>>8172 great post, really good quality. thanks for this.

chemistry is seriously cool

>>8172 Sounds like a lot of work. I'd try streaking a clean agar plate with a paper clip rubbed on the penicillin, then transferring new growth until I have a clean sample. If that doesn't work, drop a tiny flake of it on the agar. One way or another it will grow, and you'll see what else is floating around your kitchen. Using a boiled lemon as a growth medium is a terrible idea. So you boil it in water, then in open air transfer it to the container to be "inoculated" with your dirty sample? Horrifying. This will not work. Sanitizing it with alcohol will not sterilize it either, these are two different terms. Alcohol does not kill endospores. If you wanted to use a solid medium, brown rice, oats, etc would be much better choices. You hydrate it, put it in jars, then pressure cook those. That will be sterile, provided you don't open them outside of your still air box and let a bunch of stuff get in them. You are using a still air box, right lainon? However, the way they actually do it is much easier. Deep tank fermentation. At home, we call that a liquid culture, and it can be done in a mason jar. Honey and water is all you need, mix them together then sterilize. A 90 minute steam bath will be sufficient, and this technique is also sufficient for agar, so you can actually do this all without a pressure cooker. Once your LC is ready, take a little sliver of your colonized agar and drop it in. You could make gallons of the stuff. No straining bits of bread and lemon out.

>>8487 OP here. I found this recipe with a quick search on the internet, but the idea of honey, as you say, was more attractive. I'll look into this a bit. Thanks for the advice Lain.

>>8488 Check out the shroomery forums, there's lots of threads about LC. Honey should work great. One recipe I found says 285ml of water and 15g of honey. Wish I was still growing because I never tried making LC.

While diy antibiotics sounds like an interesting concept, a far easier solution would be to use the plants antibiotics are synthesized from. Medicinal herbs can be ingested and carry the effects of the antibiotics they are synthesized into. Although penicillin mold is not one of these as it's very toxic.

>>8548 >Penicillium >Mycotoxins Lain no, you can in fact eat a lot of it. Blue cheese is an example. Reaching general antibiotic potency isn't likely though. But even a little could go a long way. While on this topic >Medieval soldiers were known to carry a piece of moldy bread into battle and, were they cut, apply the heap directly to the wound as a salve While I don't believe it completely negated infection, such as fungal, bacterial sepsis is major, while lesser infections can heal over time.

>>8548 Penicillin B and G both seem to be produced from penicillium mold from what I could find from skimming their wikipedia articles. Can you provide some links about synthesizing penicillin from plants? I can't find anything about this process, as far as I know it's only produced by the mold.

>>8550 You are correct that penicillin is produced from mold. I guess I should clarify that I wasn't referring to penicillin specifically but antibios in the general sense of substances that kill bacteria actively. I believe the correct term to look for when discussing medicinal plants is if they have have antibio properties. I would say for pneumonia https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699608/ may be good depending on where you live.

Why not a ton of garlic (for allicin)??

It's a bit above the level of tech this thread was made for, but this Wolfram demo reminded me of y'all. Seeing the dynamics of a related problem can often have value, I think: twitter.com/WolframResearch/status/1521480708757327872

I'm allergic to penicillin, so I'd be glad if it left without a trace

>>8170 I admire your resourcefullness. There are times when I buy bread, let it sit too long and it looks like your picrel. Sometimes it will have black mold spots as well. I then take it, put it in a pan with a tablespoon of butter, sear/toast it. Tastes good, never get sick from it, and the bread doesn't go to waste. Is it medicinal? I don't know. I has some today with an egg and some cheese. It's food for the apocalypse!

I use the mold from limes and oranges for the heavy duty stuff. Bread mold is for dweebs. Squeezed limes can be good general non-toxic degreasers and disinfectants. soak them in water for a day or two then blend it and bottle it.

>>8572 I just recently heard about this, i think it was used in some war when lack of penicillin was a thing